Objective To establish a diabetic rat model induced by streptozotocin and evaluate its renalinjury. Method

Four-week-old male Sprague-Dawley ( SD) rats were fed a high-fat diet and injected intraperitoneally with streptozotocin ( STZ) at a dose of 30 mg / kg to induce the diabetic model. During the experiment, the general condition of the rats was observed, and fasting blood glucose ( FBG) and body mass was measured. A FBG level ≥ 11. 1 mmol / L was used as the standard for establishing the type 2 diabetes model. After successful modeling, the following parameters were assessed: 24-hour urine protein ( UP ) , urine creatinine ( UCr) , serum creatinine ( SCr ) , and blood urea nitrogen ( BUN) content. Renal tissue was collected to calculate the kidney index (KI) , and HE staining, periodic acid-Schiff ( PAS) staining, and Masson staining were performed to observe renal injury. Result

Compared with the normal group, the model group showed decreased body weight and urine creatinine levels, while 24-hour urine protein, serum creatinine, BUN content, and kidney index were significantly increased ( all P<0. 05) . No obvious abnormalities were observed in the renal pathological staining. Conclusion SD rat model of type 2 diabetes can be established through a 13-week high-fat diet and STZ injection (30 mg / kg) . This model causes renal injury; however, no pathological changes such as glomerulosclerosis, mesangial cell proliferation, or renal fibrosis were observed under light microscopy.

streptozotocin, type 2 diabetes, diabetic kidney disease, animal models

目的 通过链脲佐菌素诱导建立糖尿病大鼠模型,对其肾损伤情况进行评价。 方法 选用 4 周龄雄性 SD 大鼠通过高脂饲料喂养及腹腔注射链脲佐菌素( streptozotocin,STZ,30 mg / kg) 的方法诱导糖尿病动物模型,实验期间观察大鼠一般状态,检测空腹血糖( fasting blood glucose,FBG) 、称量体质量,以 FBG ≥ 11. 1 mmol / L 为 2 型糖尿病成模标准,造 模 成 功 后 检 测 24 h 尿 蛋 白 ( urinary protein, UP ) 、 尿 肌 酐 ( urine creatinine, UCr ) 、 血 肌 酐 ( serumcreatinine,SCr)和尿素氮( blood urea nitrogen,BUN)含量,取肾组织,计算肾指数( kidney index,KI) ,HE 染色、过碘酸雪夫( PAS)染色、马松(Masson)染色观察肾损伤情况。 结果 与正常组相比,模型组体质量、尿肌酐降低,24 h 尿蛋白、血肌酐、尿素氮含量,肾指数升高(均 P<0. 05) ,肾病理染色未见明显异常。 结论 SD 大鼠通过高脂饮食 13周及注射 STZ(30 mg / kg)的方式可建立 2 型糖尿病模型,该模型会产生肾损伤,但肾在光镜下并未出现肾小球硬化、系膜细胞增生、肾纤维化等病理变化。

链脲佐菌素, 2 型糖尿病, 糖尿病肾病, 动物模型